Composite

Part:BBa_M36081:Design

Designed by: Ashley Hammerbacher Celina Malavé   Group: Stanford BIOE44 - S11   (2014-04-30)


Tenebrio Molitor Antifreeze Protein Actuator With Secretion Tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to include the yeast secretion tag to make sure the antifreeze protein would go into the solution and exit the yeast cell.


Source

Gene of Interest from Uniprot, Kozac sequence, Terminator and Yeast secretion tag from parts registry

References